WebHowever, KRAS mutation in OVCAR-5 cells may affect AKT phosphorylation, while MEK1 mutations, in OVCAR-3 cells, may derogate the inhibitory effect on ERK1/2 phosphorylation (Table A1 and Table A2). Interestingly, both ENG-L06E and ENGA-L08E show an optimal inhibitory effect, on both ERK1/2 and AKT phosphorylation, in BRCA1-null UWB1.289 … WebDec 20, 2011 · OVCAR-5 cells were seeded in T-75 flasks at different densities (1.6 × 10 6, 1.2 × 10 6, 0.8 × 10 6 cells) with DMEM in 10% FCS, incubated for 24 h, and then treated …

Ovarian cancer cell lines derived from non-serous carcinomas

http://ezbiosystems.com/view2.asp?d_id=335 WebThe motility of OVCAR-5 and SKOV-3 cells was significantly increased in scratch wound and chemotaxis assays following treatment with recombinant versican and HA. Versican and HA also promoted invasion of SKOV-3 and OVCAR-5 cells but had no effect on OVCAR-3 … predator theory

Versican induces a pro-metastatic ovarian cancer cell behavior ... - PubMed

WebThe OVCAR-5 cell line was established from ascites fluid from a non-treated patient with an advanced-stage ovarian tumor.3 The OVCAR-5 cell line has recently been classified as … WebCellosaurus OVCAR-5 (CVCL_1628) [Text version] Cell line name OVCAR-5 Synonyms OVCAR 5; NIH:OVCAR-5; OVCAR.5; OVCAR5; Ovcar5; OVCA5 Accession … WebDec 20, 2011 · OVCAR-5 was grown to 50–60% confluency in DMEM with 10% FCS, untreated or treated with 100 μg/mL (714 nM) MIS or 60 nM doxorubicin for 48 h, harvested, and incubated with 10 μg/mL Hoechst 33342 at 37 °C for 30 min or 40 μg/mL propidium iodide (Sigma) at 25 °C for 30 min. Cells were stained with anti-human CD24-PE, anti … scoreboard free