WebH2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm). - Mechanism of Action & Protocol. From 11:00 pm to 12:00 pm EST ( 8:00 pm to 9:00 pm … WebA, cellular ROS levels in UMRC6 cells after glucose (Glc) withdrawal for 6 h were determined by H2DCFDA flow cytometer analysis. To prevent ROS generation, 2 mM NAC was added to the glucose-free...
Dcfh-DA For ROS Detection DCFHDA - Sigma-Aldrich
WebThe 2',7'-dichlorodihydrofluorescin diacetate (H2DCFDA) (Life Technologies, Eugene, OR, USA) staining for H 2 O 2 was performed as described by Yang et al. (2014). Stained roots were photographed using a laser scanning confocal microscope (LSM 710; Zeiss, Oberkochen, Germany) with excitation at 488 nm. WebDec 12, 2024 · After 24 h drug treatment, cells were incubated with 10 μM CM-H2DCFDA (Life Technologies) for reactive oxygen species (ROS) and 5 μM MitoSox Red (Life Technologies) for Mitochondrial superoxide. inherent risk factor
开环异落叶松脂素二葡糖苷(SDG)和相关化合物用于防护辐射和化 …
WebROS production was measured by the H2DCFDA assay. ... Finally, the DNA slides were dried and stained with SYBR gold (Life Technologies) as a solution (1:10,000 (v / v) in 1X Tris-EDTA (0.1 M Trisma base, 0.05 M EDTA). The following day, the cells were re-hydrated with water and fluorescent cells images, ×200 magnification (Olympus U-RFLT50 ... WebJul 9, 2015 · Cells were then trypsinized, washed once in PBS resuspended and then incubated for 30 min at 37 °C in the dark with the oxidation-sensitive fluorescent probes 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA, 10 μM) or dihydroethidium (DHE, 5 μM) (Molecular Probes, Life Technologies, NY, USA). WebAug 12, 2016 · Total ROS measurement Cells were treated as indicated, and then 10 μM 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA, Life Technologies, Cat# D-399) was added and incubated for 1 h. Excess H2DCFDA was removed by washing the cells twice with PBS. Labeled cells were trypsinized and resuspended in PBS plus 5% FBS. ml 2510 toner cartridge