Web实验七 利用FLP/FRT 重组系统获得纯合突变克隆,Mosaic杂交实验. 介绍基因功能研究策略的背景知识,以被誉为果蝇遗传学家瑞士军刀的Gal4-UAS系统原理和灵活多样的使用为 … WebDec 15, 2012 · Combination of the FLP/FRT technology with the UAS/GAL4 system allows for the control and visualization of gene expression in mosaic clones or for killing off wild-type cells such that only mutant cells contribute to the final organ or tissue of interest (Brand and Perrimon, 1993; Chou and Perrimon, 1996; Stowers and Schwarz, 1999; Pagliarini ...
mitotic clone generation using FLP-FRT system - YouTube
WebFlp-FRT系统与Cre-loxP类似,也是由一个重组酶和一段特殊的DNA序列组成。 其中,Flp(flippase recombination enzyme)重组酶,是从酵母细胞内被发现的,其基因全 … WebApr 1, 1998 · Abstract. Marked clones in mosaic animals have been used extensively to answer developmental questions in Drosophila. Recently, the use of the FLP/FRT system has allowed for the high-frequency production of mosaic animals for 95% of Drosophila genes. Cell markers have been engineered in this system to label mutant clones in both … mohawk oak oxford
条件性基因敲除的基本原理Flp/FRT系统 - 实验方法 - 丁香通
WebJan 11, 2007 · generate MARCM clones, as tubP–GAL80 will repress FLP expression. FRT site An FRTsite is a DNA sequence that, when inthe presence of FLP, will break and swap distal chromosome arms with the corresponding FRTsite. FRT sites are located close to the centromere on a chromosome arm of interest. A MARCM-ready stock will contain one … Web转基因果蝇的构建流程和如何利用平衡染色体定位转基因发生在哪条染色体上;果蝇复眼和翅(作为基本问题研究的窗口)的特点介绍等。具体实验:利用简单高效的flp/frt ... Web6 - 1 - FLP FRT 重组系统与 Mosaic 杂交实验(1)是【北京大学】遗传学实验的第29集视频,该合集共计37集,视频收藏或关注UP主,及时了解更多相关视频内容。 mohawk old quarter