Facs for mitophagy
WebAug 12, 2024 · Mitophagy is a process of selective removal of damaged or unnecessary mitochondria using autophagy machinery. Close links have been found between defective mitophagy and various human diseases, including neurodegenerative diseases, cancer, and metabolic diseases. WebApr 11, 2024 · Numerous selective autophagy pathways have been described, generally named after the cargo that is degraded—e.g., mitophagy for mitochondrial degradation and ER-phagy for endoplasmic reticulum (ER) degradation (for a review of the most recently identified selective autophagy pathways see [ 4 ]).
Facs for mitophagy
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WebMar 30, 2016 · We examined mitophagy using FACS analysis of mitochondrially targeted mKeima (mt-mKeima) (13, 32). The excitation spectrum of mKeima shifts when it is … WebMay 3, 2024 · Here, we report that protein O -GlcNAcylation, the primary nutrient sensor of glucose flux, orchestrates both ferritinophagy and mitophagy for ferroptosis. Following the treatment of ferroptosis...
WebMay 29, 2024 · Mitophagy flux compares MTDR levels with and without the mitophagy inhibitor CsA and is calculated as the ratio of MTDR accumulation after CsA treatment compared to its respective control (adapted from ref. 37). Examples of gating strategies for all of the aforementioned cytometry-based analyses are presented in Supplementary Fig. … WebSep 1, 2024 · The contribution of the Ubiquitin-Proteasome System (UPS) to mitophagy has been largely attributed to the E3 ubiquitin ligase Parkin. Here we show that in response to the oxidative stress associated with hypoxia or the hypoxia mimic CoCl 2, the damaged and fragmented mitochondria are removed by Parkin-independent …
WebOccurs via Mitophagy We addressed whether the ARIH1-mediated removal of damaged mitochondria involved mitophagy. To establish this point, we measured mitophagy using m-Keima fluorophore, a biosensor of mitochondrial degradation by the lysosomes (Katayama et al., 2011). m-Keima is a variant of RFP that is tar-geted to the … WebJun 19, 2024 · Mitophagy is a process that specifically degrades damaged or superfluous mitochondria via the autophagy-lysosome machinery. 1, 2 As a main mitochondrial …
WebMar 2, 2024 · Overexpression of AMPKα2 prevents the phenylephedrine (PE)-induced impairment of mitophagy in CMs. A. HL-1 mouse CMs were infected with an adenovirus encoding AMPKα2 and then subjected to PE (50 μmol/L) stimulation, followed by the induction of mitophagy. Mitophagy induction was performed by treatment with 20 …
Webhow to design and plan mitophagy experiments for FACS and how to perform FACS analysis to obtain the final data. BASIC PROTOCOL 1 GENERATION OF STABLE … glass globe lightWebTwo fluorescence-based imaging systems for measuring mitophagy in ESCs under mitochondrial stress. (A), Mitophagy activation in ESCs is evaluated by mt-Keima system using a FACS. ESCs stably expressing mt-Keima were treated with FCCP, rapamycin, hypoxia or starvation, and then analyzed by a FACS. glass globe hall ceiling lightWebMay 2, 2024 · Mitophagy in the absence of Ubl-phosphorylation. Mitophagy was detected by fluorescence-activated cell sorting (FACS) of untreated and CCCP-treated (20 μM for 4 h) U2OS cells containing mitochondrially targeted mKeima (mt-Keima) and transiently expressing GFP-parkin. glass globe lawn ornamentsWebMacroautophagy (hereafter referred to as autophagy) is a genetically programmed, evolutionarily conserved catabolic process that degrades cellular proteins, and damaged … glass globe for candle sconcesWebMitochondrial autophagy, also known as mitophagy, is an autophagosome-based mitochondrial degradation process that eliminates unwanted or damaged mitochondria … glass globe light fittingsWebAn increasing number of inducers are used to trigger mitophagy. These include ROS, mitochondrial toxins (FCCP/CCCP, rotenone, antimycin A, valinomycin, oligomycin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), salinomycin, and 6-hydroxydopamine), hypoxia, ceramides, and selenite. glass globe for pendant lightWebMitophagy is a selective autophagy process that specifically removes dam- agedmitochondriaviageneralautophagy.ThetwomajorrecessiveParkinson’s disease genes PINK1 and Parkin play essential roles in... glass globe light fitting