WebApr 20, 2024 · Recently, a clickable puromycin analog, O-propargyl-puromycin (OPP), was developed and applied to label the C-termini of nascent polypeptide chains (NPCs). Following affinity purification via a ... WebPuromycin is used in cell biology as a selective agent in cell culture systems. It is toxic to prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the pac gene …
Biochemical characterization of PUNCH-P. (A,B) Newly …
WebJul 15, 2024 · In addition, the method does not require special puromycin derivatives such as biotin-puromycin (Aviner et al., 2013) or clickable puromycin (Forester et al., 2024; Huang et al., 2024; Tong et al., 2024; Uchiyama et al., 2024). Our results show that pSNAP can quantify changes in NPC levels in response to environmental cues, and is useful for ... WebApr 11, 2024 · Recently, clickable puromycin reagents had been expanded to add several alkyne or azide substitutions of either the nucleoside or amino acidity moiety [19]. Another related reagent in the puromycin toolbox may be the anti-puromycin antibody, created being a rabbit polyclonal [20] initial, [21] and afterwards replaced with a industrial mouse ... maytag washing machine repair manual 16000401
Quantitative nascent proteome profiling by dual pulse
WebMore recently, clickable puromycin reagents were expanded to include various alkyne or azide substitutions of either the nucleoside or amino acid moiety [19]. Another related … WebAug 24, 2024 · Recently, a clickable puromycin analog, O-propargyl-puromycin (OPP), was developed and applied to label the C-termini of nascent polypeptide chains (NPCs). … WebSep 23, 2024 · Europe PMC is an archive of life sciences journal literature. maytag washing machine repair parts